menH [2017-06-16 20:27:46]

menaquinone biosynthesis methyltransferase

locus: BSU22750

pI: 8.17

mw: 26.43 kDa

protein length: 233 aa

gene length: 699 bp

function: biosynthesis of menaquinone

product: menaquinone biosynthesis methyltransferase

essential: no

ec: 2.1.1.163

synonyms: gerCB,menG

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.8|Biosynthesis of menaquinone]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.5|Other proteins required for biofilm formation]

Gene

Coordinates: 2,382,907 → 2,383,608

Phenotypes of a mutant

• essential [http://www.ncbi.nlm.nih.gov/pubmed/?term=12682299 according to Kobayashi et al. ]; however, inactivation reported by [http://www.ncbi.nlm.nih.gov/pubmed/?term=26735940 Meeske et al. ] and [http://www.ncbi.nlm.nih.gov/pubmed/?term=20511502 Block et al. ]
• inactivation of ''[gene|DDD40AEBBCBD3C19661A72B85912E266DBF267B3|menH]'' reduces sporulation efficiency to 12.7% that of wild type cells [Pubmed|26735940]

The protein

Catalyzed reaction/ biological activity

• S-adenosyl-L-methionine + demethylmenaquinol = s-adenosyl-L-homocysteine + menaquinol (according to Swiss-Prot)

Structure

• [PDB|1OBW] (from ''Saccharomyces cerevisiae'', 31% identity) [Pubmed|9201917]

Expression and Regulation

Operons

• genes: [gene|C45A5935559F0DAAD70F808725AF730BC18A2B0A|hepS]-[gene|DDD40AEBBCBD3C19661A72B85912E266DBF267B3|menH]-[gene|3CAEA54E80A0B56EE120B881C8CDBE055AF6BDC1|hepT]-[gene|B498ED03F19B7A73878D5C947C28C2A87CAB934D|ndk]
  description: [pubmed|22383849]

Biological materials

Mutant

• BKE22750 (*menH::erm trpC2*) available at the [http://bgsc.org Bacillus Genetic Stock Center]
• BKK22750 (*menH::kan trpC2*) available at the [http://bgsc.org Bacillus Genetic Stock Center]

References

• 9201917,26735940,20511502